Education

2015-2016

Master of Science in Microbiology from the Department of Microbiology, University of Dhaka, Bangladesh, GPA: 3.80 (out of 4.0)

Title of the M.Sc thesis (Thesis Submitted, 8 August 2017): Mutation in mgrB gene and Genetic Modification of the Structure of lipid A Exhibit Colistin Resistance in Citrobacter freundii strain NR-12 of Poultry Origin.

Supervisor: Dr. Munawar Sultana, Associate Professor, Department of Microbiology, University of Dhaka.

2011-2012

Bachelor of Science (Honours) in Microbiology with Biochemistry and Chemistry as minor subjects from the Department of Microbiology, University of Dhaka, Bangladesh. CGPA: 3.88 (out of 4.0).

2010

Higher Secondary Certificate in Science group from the Board of Intermediate and Secondary Education, Dhaka, Bangladesh. Result: GPA 5.0 (out of 5.0).

2008

Secondary School Certificate in Science group from the Board of Intermediate and Secondary Education, Dhaka, Bangladesh. Result: GPA 5.0 (out of 5.0).

Research Experiences

04/2018-Present

PI/Co-PI, Department of Microbiology at Jashore University of Science and Technology.

Projects:

Impact of Integrated Fish Farming with Livestock on Antimicrobial Resistance and Heavy Metal Bio-magnification in a Pond Environment.
Multidrug resistant biofilm forming bacteria from mobile phones of hospital personnel

Research investigator, Genome Center, Jashore University of Science and Technology.

Projects:

Bacterial 16S rRNA sequencing using Capillary Sequencing (CS) machine.
Bacterial whole genome and metagenome sequencing using Ion Torrent™ technique by Ion S5™ Sequencer.
Rapid and cost-effective method for the simultaneous genotyping of SARS-CoV-2 phylogenetic clades.
Metagenomic diagnosis and pathogenic network profile of SARS-CoV-2 in patients co-morbidly affected by type 2 diabetes.
Genomic analysis of SARS-CoV-2 variants of concern identified from the ChAdOx1 nCoV-19 immunized patients from Southwest part of Bangladesh

08/2017-04/2018

Research Assistant in Microbial Genetics and Bioinformatics Laboratory, Department of Microbiology at University of Dhaka, Bangladesh.

(Project: Isolation of multidrug resistant Salmonella spp. from the poultry sources such as meat, egg, droppings and cloacal swab.)

06/2016-08/2017

Thesis student, Department of Microbiology, University of Dhaka, Bangladesh.

(Project: The prevalence of colistin resistant Enterobacteriaceae in poultry)

Research Projects and Grants

Metagenomic analysis of integrated fish pond revealed the involvement of genes conferring co-selection to heavy metal and antibiotic in microbial community. Funded by: Jashore University of Science and Technology; 2020-2021
Molecular detection of multidrug resistant bacteria from pediatric pneumonia patients of Khulna division. Funded by: Jashore University of Science and Technology; 2020-2021
Impact of Integrated Fish Farming with Livestock on Antimicrobial Resistance in a Pond Environment of Khulna Division. Funded by: Ministry of Science and technology under special allocation for fiscal year 2019-20.
Molecular Characterization of multidrug resistant bacteria isolated from mobile phones of hospital personnel in Khulna division of Bangladesh. Funded by: R&D project for the year 2019-2020 under the Ministry of Science and Technology, People’s Republic of Bangladesh.
Isolation of multidrug resistant biofilm forming bacteria from mobile phones of hospital personnel in Khulna division of Bangladesh. Funded by: R&D project for the year 2018-2019 under the Ministry of Science and Technology, People’s Republic of Bangladesh.

Peer-reviewed publications:

Al-Emran, H. M., Rahman, F., Sarkar, L., Das, P. K., Mondol, P., Yesmin, S., Sultana, P., Ahammed T., Parvez, R., Hasan, M. S., Sarkar L. S., ... & Hossain, M. A. (2024). Emergence of SARS-CoV-2 Variants Are Induced by Coinfections With Dengue. Bioinformatics and Biology Insights, 18, 11779322241272399.
Hasan, M. S., Rahman, M. S., Das, P. K., Alam, A. R. U., Islam, O. K., Al-Emran, H. M., ... & Jahid, I. K. (2024). Alternative genome sequencing approaches of SARS-CoV-2 using Ion AmpliSeq Technology. MethodsX, 12, 102646.
Hasan, M. S., McElroy, K. E., Audino, J. A., & Serb, J. M. (2024). Opsin expression varies across larval development and taxa in pteriomorphian bivalves. Frontiers in Neuroscience, 18–2024. https://doi.org/doi: 10.3389/fnins.2024.1357873.
Meghla, N. S., Kobir, M. M., Rashid, M. O., Sakib, N., Hasan, M. S., Islam, M. T., & Islam, O. K. (2024). Pediatric pneumonia patients in Jashore city, Bangladesh: screening of drug resistance pattern and molecular identification. Bangladesh Journal of Medical Science, 23(3), 729-738.
Hasan, M. S., Chowdhury, S. R., Meghla, N. S., Sakib, N., Shah, S. T., Islam, M. T., & Islam, O. K. (2023). Molecular typing of multidrug resistant bacteria isolated from health care professionals’ mobile phone: A pilot study in Jashore, Bangladesh. Bangladesh Journal of Medical Science, 22(3), 643-656.
Al‐Emran, H. M., Rahman, S., Hasan, M. S., Ul Alam, R., Islam, O. K., Anwar, A., ... & Hossain, A. (2023). Microbiome analysis revealing microbial interactions and secondary bacterial infections in COVID‐19 patients comorbidly affected by Type 2 diabetes. Journal of Medical Virology, 95(1), e28234.
Rahman, M., Abdullah-Al-Mamun, M., Khatun, M., Khan, A. S., Sarkar, O. S., Islam, O. K., Sakib, N., Hasan, M. S., Islam, M. (2022). Contamination of Selected Toxic Elements in Integrated Chicken-Fish Farm Settings of Bangladesh and Associated Human Health Risk Assessments. Biological Trace Element Research, 1-13.
Shah, S. T., Akter, K., Hasan, M. S., Chakrovarty, T., Sakib, N., & Islam, M. T. (2022). Factors determining preventive practices of the healthcare workers regarding COVID‐19 in Bangladesh. Health science reports, 5(1), e484.
Al-Emran, H. M., Hasan, M. S., Setu, M. A. A., Rahman, M. S., Alam, A. R. U., Sarkar, S. L., ... & Hossain, M. A. (2021). Genomic analysis of SARS-CoV-2 variants of concern identified from the ChAdOx1 nCoV-19 immunized patients from Southwest part of Bangladesh. Journal of infection and public health.
Rubayet Ul Alam, A. S. M., Islam, O. K., Hasan, M. S., Islam, M. R., Mahmud, S., Al‐Emran, H. M., ... & Anwar Hossain, M. Dominant Clade‐featured SARS‐CoV‐2 Co‐occurring Mutations Reveals Plausible Epistasis: An in silico based Hypothetical Model. Journal of Medical Virology.
Hasan, M. S., Islam, M. T., Alam, A. R. U., Sarkar, S. L., Rahman, M. S., Islam, O. K., ... & Hossain, M. A. (2021). Initial reports of the SARS‐CoV‐2 Delta variant (B. 1.617. 2 lineage) in Bangladeshi patients: Risks of cross‐border transmission from India. Health science reports, 4(3).
Islam, M. T., Alam, A. R. U., Sakib, N., Hasan, M. S., Chakrovarty, T., Tawyabur, M., ... & Anwar Hossain, M. (2021). A rapid and cost‐effective multiplex ARMS‐PCR method for the simultaneous genotyping of the circulating SARS‐CoV‐2 phylogenetic clades. Journal of medical virology, 93(5), 2962-2970.
Biswas, S., Al Saber, M., Tripty, I. A., Karim, M. A., Islam, M. A., Hasan, M. S., Ul Alam ASMR,... & Hasan, M. N. (2020). Molecular characterization of cellulolytic (endo-and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh. Annals of Microbiology, 70(1), 1-11.
Islam, O. K., Al‐Emran, H. M., Hasan, M. S., Anwar, A., Jahid, M. I. K., & Hossain, M. A. (2021). Emergence of European and North American mutant variants of SARS‐CoV‐2 in South‐East Asia. Transboundary and emerging diseases, 68(2), 824-832.
Hasan, M. S., Sultana, M., & Hossain, M. A. (2019). Complete genome arrangement revealed the emergence of a poultry origin superbug Citrobacter portucalensis strain NR-12. Journal of global antimicrobial resistance, 18, 126-129.

Conference Presentations/ Workshops

Attending a 4 days training program on “Microbial Community Analysis Using 16S Metagenome Approach”, conducted by Chaperone, Bangladesh, 11-19 March 2022.
Attending an 8 days training program on “Application Training on NGS & CS”, conducted by Thermo Fisher Scientific, India, 17-25 March 2019.
Attending a 4 days training course on “Real Time PCR, Capillary and Next Generation Sequencing Technology” conducted by Genome Center, Jashore University of Science and Technology, 18-21 November 2018.
Attending on the 30th Annual Conference of Bangladesh Society of Microbiologists (BSM) at Nabab Nawab Ali Chowdhury Senate Bhaban, University of Dhaka, April 29, 2017 and I presented a poster on the topic “Screening and Detection of Colistin Resistance in Citrobacter and Proteus spp. of Poultry Origin”.
Attending on a national conference on “Biochemistry and Molecular Biology for Life Sciences”, December 10, 2016, organized by “The Bangladesh Society for Biochemistry and Molecular Biology in association with Department of Biochemistry and Molecular Biology, University of Dhaka” and I presented a poster on the topic “Characterization of Colistin Resistance in Enterobacteriaceae of Poultry Origin”.
Attending the thirty-five hours training course on “Statistical Data Analysis for Biological Sciences” conducted by Institute of Statistical Research and Training (ISRT), University of Dhaka, under HEQEP project (CP#3236), 2016.
Attending Workshop on Bioinformatics and Whole genome analysis by Microbial Genetics and Bioinformatics Laboratory, Department of Microbiology at University of Dhaka, 2016.
A poster was presented, titled “Genotypic Diversity of Antibiotic Resistant Poultry Salmonella spp. in Bangladesh” in the 3rd AFSA Conference on Food Safety and Food Security at KIIT University, Bhubaneswar, India, September 16, 2016.
Attending a 15 days internship program as undergraduate project conducted by GlaxoSmithKline Bangladesh Limited, 2015.
Attending Workshop on “Pasteurization and commercial processing of milk” conducted by Milk vita factory Limited, Bangladesh, 2014.

Teaching Experience

December 2020-Onwards

Assistant Professor, Department of Microbiology, Jashore University of Science and Technology

*Taught courses like microbial biotechnology, virology, food and dairy microbiology, biosafety and biorisk management in undergraduate level.

*Created course materials, assignments, exam and graded works.

April 2018-December2020

Lecturer, Department of Microbiology, Jashore University of Science and Technology

*Worked as a member in the department curriculum and syllabus development committee

Relevant Skills

Genomic DNA/RNA and Plasmid DNA isolation, PCR/qPCR/Reverse transcriptase PCR, RAPD, ARDRA, Agarose Gel electrophoresis.
Microscopy such as Light, Dark Field, Flurescence and Field Emission Scanning Electron Microscopy (FE-SEM)
Instrument operations skills such as Sanger Sequencer- SeqStudio, Next Generation Sequencing (NGS)- Ion GeneStudio S5, Field Emission Scanning Electron Microscope- Zeiss Sigma 500VP
Raw DNA Sequence cleanup, Molecular Phylogeny analysis, Primer designing, Protein Modeling, Molecular docking, Dendrogram and Cluster analysis, Whole Genome Sequence data analysis and use of various Bioinformatical tools.

Academic Awards and Scholarships

National Science and Information & Communication Technology Fellowship, 2016 from Ministry of Science and ICT, as a recognition of my thesis work.
Dean’s Award 2015 from Faculty of Biological Sciences, University of Dhaka, as recognition of outstanding result in the BS (Honors) Examination.
Received Board scholarship in Higher Secondary Certificate (Science), 2010 from the Ministry of Education, Bangladesh.

EDUCATION

Md. Shazid Hasan

Md. Shazid Hasan

Dept. of Microbiology

Jashore University of Science and Technology

Bachelor of Science (Honours) in Microbiology

Duration: 2012 - 2015

Master of Science in Microbiology

Duration: 2016 - 2016

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